Analysis Note
Negative ControlSK-OV-3 cells or normal skin tissue
Positive ControlA431 cells or breast carcinoma tissue
Application
Frozen sections (1 g/ml; see application references)Immunoblotting (0.1-1 g/ml; see application references)Immunocytochemistry (1-2.5 g/ml; see application references)Immunoprecipitation (1 g/ml or use Cat. No. OP43A; see application references)Paraffin sections (1 g/ml, pepsin, heat or pressure cooker pre-treatment required; see application references)
General description
Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X63Ag8.653 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).
Immunogen
wild type, recombinant, human p53
Human
Epitope: Within amino acids 21-25 of human p53
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Recognizes both mutant and wild-type p53 under denaturing and non-denaturing conditions. This antibody reacts weakly with rodent p53; we do not recommend it for rodent samples. For gel shift assay, use Cat. No. OP43L resuspended in 100 µl of buffer. Wild-type p53 has a short half life and is present in low amounts in cells. For immunoprecipitation increasing the amount of sample and labeling with 35S-Met for less than or equal to 1 h will aid in visualizing wild-type p53. Antibody should be titrated for optimal results in individual systems.
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.Legros, Y., et al. 1994. Oncogene9, 2071.Barak, Y., et al. 1993. EMBO J.12, 461.Kastan, M.B., et al. 1992. Cell71, 587.Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.Lane, D.P. 1992. Nature358, 15.Vojtesek, B., et al. 1992. J. Immunol. Meth.151, 237.Kastan, M.B., et al. 1991 Cancer Res.51, 6304.
Packaging
Please refer to vial label for lot-specific concentration.
20, 100 µg in Plastic ampoule
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Warning
Toxicity: Standard Handling (A)
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